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Item Q07
�S Q.7 I`� County of Monroe �y,4 ' �, "tr, BOARD OF COUNTY COMMISSIONERS County �a� Mayor Michelle Coldiron,District 2 �1 nff `ll Mayor Pro Tem David Rice,District 4 -Ile Florida.Keys Craig Cates,District 1 Eddie Martinez,District 3 w � Holly Merrill Raschein,District 5 County Commission Meeting November 17, 2021 Agenda Item Number: Q.7 Agenda Item Summary #9709 BULK ITEM: No DEPARTMENT: Sustainability TIME APPROXIMATE: STAFF CONTACT: Rhonda Haag (305)453-8774 10:45 A.M. AGENDA ITEM WORDING: Presentation by the University of Miami on the results of two years of canal and near shore monitoring to measure the effects canal water quality may have on nearshore waters. ITEM BACKGROUND: This item is for the University of Miami ("UM") to present the results of two years of canal and nearshore monitoring. This work is separate from the Florida Keys Reasonable Assurance Document ("RAD") Monitoring work also conducted by the University of Miami on behalf of the Department of Environmental Protection. The monitoring started in July 2019 and ended in June 2021. UM sampled surface water quality on a quarterly basis in 7 residential canals and in 4 "non-canal" transects throughout the Florida Keys, collecting 84 samples per quarter (336 samples in a calendar year). In addition, biotic sampling occurred twice a year at all the water quality sampling sites to document biological diversity in and around the canal environs. The question addressed in this scope of work was "Do canals in the Florida Keys contribute to nearshore water quality degradation?" This task addressed this question broadly by looking at 9 canals throughout the Florida Keys and comparing canal assessment to underdeveloped shorelines ("non-canals")primarily in parks and protected areas. The operation premise in RAD assessments is that RAD water quality stations located 500 meters from shore are at the limit of land-based sources of pollution (e.g. from run-off and canals), and thus represent an assessment of regional (vs. local) influences on nutrient loading. The work examined the "gap" from the canals to the 500-meter RAD stations by sampling across a randomized block design of the shoreline to 500 meters offshore gradient. This study addressed the potential contributions of local vs. regional factors in degradation of near shore water quality after the compliance Keys-wide of advanced wastewater treatment. Packet Pg. 1426 Q.7 Sampled Areas Legend /'\ State Park Study Areas A Canal Study Areas i !o d S rnV�llt c Cn'an rho Tamp r' it 11 0246810 20 30 40 Kilometers r 012345 10 15 20 Miles Esn HERE tea co n t�pgn�tr etMap aarin6utars p P and the GIS;user community Figure 1: Maps of 13 locations with designations as canals or "non-canals". Yellow points are transects of canals (N=9); Green points are transects of non-canal coastal areas (N=7). TABLE 1: LIST OF WATER QUALITY PARAMETERS Packet Pg. 1427 Q.7 1 u, t r,�� �1 t ,c> St\y , ,,,y )\\S\r,,s4>u , ,}, y ,i)�\ r,, ),aoltll Yt.,,,f), ,,r ,,�,,, r(S\{,I v,,,, t1,\t i ti,,, ,f ,s4,} ,,\ .j, r�'„t s\�„•;:d NO. Water Grab Nitrite+Nitrate in aqueous EPA 353.2 Rev. 2.0 0.004 mg/L matrices as mg/L as N TP Water Grab Total Phosphorus in aqueous EPA 365.1 Rev. 2.0 0.002 mg/L matrices as mg/L as P TKN Water Grab Total Kjeldahi Nitrogen in EPA 351.2 Rev. 2.0 0.08 mg/L aqueous matrices as mg/L as N Phytoplankton chlorophyll-a Chlorophyll a Water Grab (corrected for phaeophytin)and SM 10200 H(mod.)) < 1.0 ug/L phaeophytin by spectrophotometry Dissolved oxygen(DO) Dissolved Field Discrete Not Oxygen Measurement concentration in water Measurement applicable measured by field meter Percent DO Field Percent DO saturation in water Discrete Not Saturation Measurement measured by field meter Measurement applicable H Field pH level in water measured by Discrete Not p Measurement field meter Measurement applicable Specific Field Specific conductance of water Discrete Not Conductance Measurement measured by field meter Measurement applicable Water Field Water temperature measured by Discrete Not Temperature Measurement field meter Measurement applicable Packet Pg. 1428 Q.7 t z a a �I! ➢ hw4a — ' W, . IF i to s' v f?i� y i'11 R aa[ C.ti> i Fixed Points Zone 50 100 200 300 400 500 Meters Zone A Points A t C; Zone B Points KM B Zone C Points C a,urce-: sri, C?iital �, ee 1_ye �arhs ar' � �, �eagr�phi�s C�J� IAirbus L�S�i���_1:Sa��.��lS� �,er� €�IC�a Figure 2: Example 200 meter by 500 meter sampling grid established for all 13 survey sites. This example is Canal 28 in Key Largo. Samples were taken on outgoing tides. PREVIOUS RELEVANT BOCC ACTION: 08/16/17: Approval to advertise an RFP for 2 years of water quality monitoring services in support of the Florida Keys Reasonable Assurance Document (FRAD) as requested by the Department of Environmental Protection (DEP), 50% funding to be provided by DEP and 50% match required by the County and municipalities, exact match amount to be determined after solicitation opening, estimated in the range of$50,000-$75,000/year, which is not budgeted. 05/15/18: Approval to negotiate with vendors in rank order for nearshore and canal water quality monitoring services in support of the Florida Keys Reasonable Assurance Document(FRAD). 08/15/18: Approval to enter into a $360,718.00 Agreement with the University of Miami for water quality monitoring services in support of the Florida Keys Reasonable Assurance Document(FRAD) with the Department of Environmental Protection (DEP), 50% funding to be provided by DEP through Grant Agreement MN008 and 50% funding cost share provided by the County and municipalities. CONTRACT/AGREEMENT CHANGES: N/A Packet Pg. 1429 Q.7 STAFF RECOMMENDATION: N/A DOCUMENTATION: 1 st Amendment 05/22/2019 FINANCIAL IMPACT: Effective Date: N/A Expiration Date: October 15, 2021 Dollar Value of Work: $497,211 Budgeted: N/A Original Source of Funds: 304 Funds CPI: No Indirect Costs: Estimated Ongoing Costs Not Included in above dollar amounts: None Revenue Producing: No If yes, amount: Grant: No. County Match: N/A Insurance Required: No Additional Details: 11/17/21 304-23000 - PHYSICAL ENVIRONMENT $0.00 REVIEWED BY: Rhonda Haag Completed 10/27/2021 9:39 AM Cynthia Hall Completed 10/27/2021 4:35 PM Purchasing Skipped 10/27/2021 4:35 PM Budget and Finance Completed 10/27/2021 4:40 PM Maria Slavik Completed 10/27/2021 5:42 PM Liz Yongue Completed 11/02/2021 9:51 AM Board of County Commissioners Pending 11/17/2021 9:00 AM Packet Pg. 1430 p�R 60URr4 Q.7.a. °°�° °°f Kevin Madok, CPA Q 5 Q •A i o ...... Clerk of the Circuit Court& Comptroller—Monroe County, Florida N Rae coup• m DATE: July 23, 2019 y TO: Rhonda Haag, Director Sustainability&Projects Lindsey Ballard,Aide to die County Administrator CL FROM: Pamela G. HancocC. SUBJECT: May 22' BOCC Meeting Attached is an electronic copy of the following item for your handling: 06 Amendment No. 1 to die Agreement with die University of Miami, to provide canal and nearshore water monitoring services to measure die effects canal water quality may have on nearshore waters, in the amount of$497,211.00. e Should you have any questions please feel free to contact me at(305) 292-3550. 0 LO N N cc: County Attorney Finance File KEY WEST MARATHON PLANTATION KEY PK/ROTH BUILDING 500 Whitehead Street 3117 Overseas Highway 88820 Overseas Highway 50 High Point Road Key West,Florida 33040 Marathon,Florida 33050 Plantation Key,Florida 33070 Plantation Key,Florida 33070 305-294-4641 305-289-6027 305-852-7145 305- Packet Pg. 1431 Q.7.a AMENDMENT ONE (1) TO CONTRACT BETWEEN MONROE COUNTY BOARD OF COUNTY COMMISSIONERS AND y UNIVERSITY OF MIAMI c FOR FLORIDA KEYS WATER QUALITY MONITORING SERVICES This AMENDMENT ONE (1) TO CONTRACT ("Amendment") is entered into on the 22nd day of May, 2019, to that contract dated July 18, 2019, between the Monroe County Board of County Commissioners, 1100 Simonton Street, the Gato Building, Room 2-205, Key West, Florida 33040, (COUNTY) and the University of Miami, 1320 S. Dixie Highway, Suite 650, Coral Gables, FL 33146 (UNIVERSITY). WITNESSETH c WHEREAS, the .COUNTY and UNIVERSITY entered into a certain contract dated July 181h, 2018 ("Agreement")to perform water quality monitoring services for the update to the Florida Keys Reasonable c Assurance Document (FRAD) and to perform Canal Monitoring services for the COUNTY; and WHEREAS, the Florida Department of Environmental Protection (DEP) via a DEP Grant Agreement (Agreement Number MN008) provides funding to Monroe County for the performance of water quality monitoring services to update the FRAD (Task 1). This DEP Grant Agreement (Agreement Number MN008) includes Monroe County funds via a cost-share to support Task 1. The Agreement between COUNTY and UNIVERSITY in turn provides the funding to UNIVERSITY to perform Task 1; and WHEREAS, the parties now desire to amend the Agreement pursuant to the terms and conditions as set forth herein to include a task (Task 2) to measure the effects that canal water quality may have on near LO shore waters quality; and WHEREAS, Monroe County will provide funding for the performance of Canal Monitoring services (Task 2). NOW THEREFORE,in consideration of the mutual promises and covenants contained herein, it is agreed as follows: 1. Section 2: SCOPE OF THE WORK is hereby revised to include the Task 2 as reflected in the attached Exhibit A-1. 2. Section 3: CONTRACT AMOUNT is hereby revised to include additional funding to support Task 2. The COUNTY shall pay the UNIVERSITY an additional amount not to exceed Four Hundred and Ninety-Seven Thousand Two Hundred Eleven Dollars ($497,211.00) as reflected in the attached budget at Exhibit C-1. The funds for this increase are Monroe County funds. The total Packet Pg. 1432 Q.7.a amount to be paid to UNIVERSITY for the work performed under this contract shall not exceed Eight Hundred and Fifty-Seven Thousand Nine Hundred and Twenty-Nine Dollars($857,929.00). 3. Section 5: TERM OF AGREEMENT is hereby revised to extend to October 15,2021.The'revised Agreement term is August 15, 2018 through October 15, 2021 for work performed under Task 2. y 4. Except as expressly provided in this Amendment, all other terms, conditions and provisions of the Agreement shall apply and remain in full force and effect. SIGNATURE OF THE PERSON EXECUTING THE DOCUMENT MUST BE NOTARIZED y TNESSED BY ANOTHER OFFICER OF THE ENTITY. CL MONROE COUNTY BOARD OF COUNTY COMMISSIONERS r Q By: De uty Clerk Mayor Sylvia Murphy N Zee! � Date 22� c). E -"r— 0 (SEAL) ,n, ���. a' UNIVERSITY OF MIAM�a�':X, w o Attest: By: By: Print Name:ch��(MG�{%l �2�5 � Print Name: Barbara A140Ie N _ cc-- 11 1 MsWate Vice President for Re§� d Admhlshl]X Title:J(• .ut 60 fit uu+�. t/6i�t Title: cv Date: Date: 671• /q N cv STATE OF FLORIDA COUNTY OF On this day of , 20 ICy before me ,the undersigned notary public, personally appeared ._k 13 � ` 6 ::known to me to be the person whose name is subscribed above or who produced s identification, and acknowledged that he/she is the person who executed .he a ve contract with Monroe County for the purposes therein contained. Notary Public BIME.RIWM `0. Expttas 0c�"11,2022 gppg66d010 Print Name ,,,,o, eadadTW'rmyF*k"" OE CM ORNEY My commission expires: za pp p P D OJ.M A TY TT RNEI Date Packet Pg. 1433 Q.7.a EXHIBIT A-1 AMENDMENT TASK 2—SCOPE OF WORK: Residential Canal'WO Impacts on Near Shore Environs y A summary of the overall costs are: TASK 2:Residential YEAR 1 YEAR 2 YEAR 3 YEAR 4 Not to Exceed total Canal WQ impacts on 2019 2020 2021 (6/1/21- c Near Shore Environs 10/15/21 WQ, BENTHIC SAMPLING, and $0 $279,103 $200,933 $17,175 $497,211 y EXTREME EVENT 2 PROJECT OVERVIEW AND RATIONALE _ As a separate effort under Task 1, Monroe County contracted with the University of Miami Coastal . Ecology Laboratory to carry out nearshore water quality monitoring at 65 stations throughout the Keys. in a partnership with the Florida Department of Environmental Protection (FDEP). The scale of watersheds makes interrelationships between the, hydrological cycle, plant diversity and natural communities easily perturbed by land-use changes on small islands. Patterns of human development in the Florida Keys have had immense impacts on ecosystem function, and have historically accelerated the amount of freshwater and nitrogen entering the hydrological cycle. Some of these trends have been reversed recently with restoration and remediation efforts. Small islands allow nutrients and sediment to enter near shore waters through groundwater seepage and surface storm run-off(Aronson et al., 2003). Tropical carbonate islands such as the Florida Keys once U relied on dense broadleaf forests and mangrove wetlands to restrict nutrient input to marine environments, supporting clear turquoise waters indicative of oligotrophic conditions. The Florida Keys now has about c 500 dredged canals of varying depths, lengths, and orientations. Canals can trap organic material, including seaweed and seagrass, which accumulates and contributes to poor water quality with the accumulation of nutrients. Poor design and circulation in canals have been addressed in the past through a series of demonstration projects to remediate this problem t1frough back-filling, adding culverts, or adding seaweed curtains. Universal wastewater treatment has been implemented throughout the Florida Keys, removing cesspits and septic systems as a source of land-based sources of nutrients. However, the LO legacy of the past rests at the bottom of dredged canals ("Eutrophication in coastal canals," 1972). Nearshore marine communities are the downstream recipients of freshwater and nutrients from island hydrological cycles. Changes in island hydrology have potential repercussions for near shore habitats as they have been the most acutely impacted by eutrophication with the extirpation of invertebrate species and dramatic changes in the ecological community composition (De Carlo, Hoover, Hoover, Young, & Mackenzie, 2007; Lapointe & Matzie, 1996; Wagner, Mielbrecht, & van Woesik, 2008; Wolanski, Martinez, &Richmond, 2009). The question to be addressed in this scope of work ("Task 2") is, "Do canals in the Florida Keys E contribute to nearshore water quality degradation?"The scope of work over two years should provide a "Yes", or "No" answer to this question with supporting data and analyses. The challenge is to design an efficient and cost-effective plan to understand any. "halo" effect of nutrients from canals moving into adjacent nearshore environments. Task 2 aims to address this question broadly by looking at 9 canals throughout the Florida Keys and comparing canal assessment to underdeveloped shorelines("non-canals")primarily in parks and protected Packet Pg. 1434 Q.7.a areas. The operation premise in FKRAD assessments is that Task 1 water quality stations located 500 meters from shore are at the limit of land-based sources of pollution (e.g. from run-off and canals), and thus represent an assessment of regional (vs. local) influences on nutrient loading. Task 2 will examine the "gap" from the canals to the 500 in Task 1 stations by sampling across a randomized block design of the shoreline to 500 in offshore gradient. Task 2 is a "stand-alone" project designed to specifically address the question of residential canal Ch contributions to nearshore water quality. This study will address the potential contributions of local vs. regional factors in degradation of near shore water quality after the compliance Keys-wide of advanced 0 waste water treatment(Figure 1). e Figure 1: Timetable of the major policies and reports that impact Florida Keys wastewater transmission y and treatment (Barreras, Kelly, Kumarb, &Solo-Gabriele, 2019). 2 19701S . . . . . . . . Cesspits are the major method of vastewater treatment and disposal= >1.970 . . . . . . . . . Beginning in the 1970s,on-site wastewater treatment systems(OWTS) began to appear in the Keys 1 410 In 1991 -1995 Monroe County adopted its Comprehensive Plan pursuant to rule 28-20 of the Florida Administrative Code (FA_C_) "Work Program" to promote wastewater facilities to 0 meet advanced wastewater treatment (AWT) or best available technology (BAT)standards to reduce nutrient loading to nearshore waters= C 1997. . . . . . . . . . The Florida Legislature sets Advanced Wastewater Treatment (AWT) effluent standards 3 0 1999, . . . , . _ . _ . . Monroe County re-instates cesspool identification and elimination program 3 0 2000. . . . _ . _ _ _ . _ Chapter 2010-20S requires Monroe County, each municipality, and those special districts responsible for wastewater treatment to r- complete the projects detailed in the Wastewater Master Plan (2000)- LO N LO 2-005. . . . . . . . . . . November 2005, The Village of Islamorada Wastewater Management Master Plan is implemented s The new treatment plant at Key Largo went 2010. . . . . _ � into operation in 2010 and is designed to meet Florida's advanced water treatment (AWT) requirements 6 r 2013. . . . . . . . . . .410 The 2013 State Budget included $50 M for Keys Wastewater projects ° 0 2015. . . . . . . . . . The Florida legislature established a deadline of December 31, 2015 for all septic tanks,cesspits, U private package plants, and central sewer systems within the Florida Keys to meet advanced wastewater treatment standards 7 Packet Pg. 1435 Q.7.a TASK 2 DESCRIPTION: RESIDENTIAL CANAL IMPACTS ON NEARSHORE WATER QUALITY CANAL WATER QUALITY STUDY: The Task 2 is.designed to answer specific questions relating to the impact of residential canals on the near W shore water quality of the Florida Keys, regardless of canal design, size and orientation. The questions to be answered include: o 1. Is there a difference in water quality between nearshore waters adjacent to canals, and nearshore o waters not adjacent to canals, and does the distance from shore (zone) have an effect (up to the 500m distance from shore)? 2. Is there a difference in the number of and diversity within marine habitat types (e.g. CMECS biotopes 1)between canal and non-canal nearshore environments, and does the distance from shore (zone) have an effect (up to the 500m distance from shore)? 3. Is there a difference in the epifauna community composition and diversity between canal and non- canal nearshore waters, does the distance from shore matter, and how does the epifauna composition and diversity compare to a reference expectation? A STANDARD QUALITY ASSURANCE PLAN (QAPP) DOCUMENT will be submitted as per the FDEP protocols and reviews for this task. The task will include the collection of water quality and benthic data; all water quality data collected for this contract will need to be uploaded quarterly by the contractor into the Department's Watershed Information Network (WIN) database. This task will sample surface water quality on a quarterly basis in 9 residential canals and in 4"non-canal" sites throughout the Florida Keys, collecting up to 15 samples per site for each quarter. Biotic sampling will occur twice a year (wet season/ dry season) at all the water quality sampling stations to document o biological diversity and natural community classification in and around the canal environs. In addition, surface water samples will be collected at some stations up to 48 hours after an extreme rainfall or storm W event to document event impactsZ, up to 187 additional water quality samples in one year. N TASK OVERVIEW: Start Date: 1 May 2019 LO End Date: 15 October 2021 Number of Sites = 13 a 9 canal sites E • 4 non-canal sites Number of Site Water Quality Samples: • 9 canal sites with 15 samples per canal block array • 4 non-canal sites with 13 samples per non-canal block array (no samples taken inside canals) Coastal and Marine Ecological Classification Standard (CMECS), see https:Hiocm.noaa.gov/cmecs/ 2 Extreme events can be defined by a specific meteorological trigger,for example,over 2" of rainfall in on 24 hour period is extremely rare,and has occurred only twice in Key West over the past 10 year(apart from direct hurricane impacts). Packet Pg. 1436 Q.7.a TOTAL WATER QYUALITY SAMPLES per QUARTER= 15*9= 135 (canal samples)+4* 13 =53 (non-canal samples) = 187 ONE EXTREME RAINFALL EVENT SAMPLING =up to 187 samples (optional) Estimated total number of Canal Monitoring Water Samples per year: 748 to 935 samples (187 stations sampled 4 times each calendar year, plus an optional extreme event sampling). 0 Biodiversity Assessment=All 13 sites surveyed twice annually,Biological Assessments will be carried c out at the same sites, with 6 stations within sites sampled twice a year (84 * 2 = 168 benthic surveys annually). Benthic stations are a subset of the water quality stations. y Task 2 will: ' 1. Perform comprehensive water quality nutrient data monitoring of 13 sites with block design sampling _ to support the County's canal program to determine the connection and impact of canals on the nearshore waters with quarterly sampling, 2. Report and upload to Florida's Watershed Information Network (WIN) the water quality data collected, 3. Perform an ecological survey of benthos along the same 13 sites with bloc design sampling to determine the response of biotic communities to water quality with sampling twice a year(wet season/ dry season), and 4. Perform comprehensive nutrient data monitoring at water quality stations after an extreme rainfall 76 event for up to 187 stations in a calendar year. Task 2a: Water Quality Block Design Description c Sampling Area Design Water samples will be collected in a "block design," using stratified random sampling of a series of stations that exist within a total sampling area that extends 500 meters out-from the mouth of the canal and 100 meters parallel to the shoreline in both directions. This will result in a 500 m X 200 m area which will be divided into 50 m X 50 m blocks.The sampling area is divided into 40 blocks.The overall sampling LO area will be divided into three zones which will extend perpendicular from the shoreline. Zone 1 will extend from the mouth of the canal to 100 m from shore. Zone 2 will extend from the edge of Zone 1 out another 200 m from shore and Zone 3 will extend from the edge of Zone 2 out an additional 200 m (to 500 m from shore). See Figure 2 below for an example of sample area design in Key Largo Packet Pg. 1437 Q.7.a Legend Sample Area Zone) N, Cna .. Cn CL 1 i C 4 0 d . 0 Figure : Illustration of th e b lock deslcgn to s a mple wa of r-quolit frcaM inshore to affsh ore from canal openings in the Florida Keys. /\ rid will be establishedin GIS that will extend 200 meters p rpendicuirrr to the raroarth of the con 1, and 500 meters offshore. 0 ScrrrrlV nag Area Design Block sampling will be completed in two types ofareas: canals (N ) and restored or intact coastlines ( =1) to allow liar e-trmparison between the canal and ` non-canal" environs. 'File annUal tnorntoring will consist ofar1 estillpated 1 S7 samples per quarter chviclecl across 13 study sites with Lip to 15 samples per- site: per-gUarter; Using the following, distribution (See Figurc 3); LO 1 sample from the rnidl.)oint ofthe carnal B. 1 sample from the 11101,101 of the canal E C� 2 samples from the offshore corners, ofthe sampling area (100 r11 parallel to the month of,canal, 500 meters perpendicular to the r11c11.r111 of caanal) 11 ;, samples from the inshore corners ofthe sampling area (100 m parallel tO nlcacrth of carpal, as faar in share as is accessible by sarlaplin vessel) p`. i samples from randomly selected blocs ir1 Zone 1 � 1`. � samples I Qom r€rrlcicamly selected blocks in Zone ? � G. I samples l 'om rancic1r111y selected blocks in Zone ''knurl-carnal sites will have 1 I stations. lackinL, the ? stations. at the midpoint and r1lcluth of the canal. Packet Pg. 1438 Q.7.a Legend Sample Locatio A � t 0 � r �# n 21 'i A V Sample Area 0 Zone it CU i 2 = CL ti4 .E 0 6 -r, Figure, : Blacks will be selected randomly for each graarterly sampling event, with a total of three � saaples taken in zone. Sample locations - correspond to the list weave. c ra c� ,1_11e sample locations Nvi11 reCIUire a boat for accessihi1ity and three staff per sampiin tears. The sampling �rrideliraes are as f 611 \vs: a. Collect samples only on outs#roue tides- while water{ is leaving the. canal. 1). Ensure wind is not iraapedint, water Irom flowing out ofthe carnal, with collection of CN samples at mid-depth if wind is are issare. LO laatea7?01aaticara 0/(/afro The data from the 15 samples "Ill he interpolated to create ar raster strr',l'acc to estin-late the \Hater gUalita pararneter°s across the entire sampling :area using a spline interpolation and a grid, size ral`5 meters. See n Figure 4 for example of"iriteg.)o1ated data surface. Table 1 iraclUdes a list of water quality parameters which rill be assessed at each san-ijiling, station within the sampling area. � Packet Pg. 1439 Q.7.a Table 1: List of water quality parameters to be assessed at each station Vaaraaaio r Saa III I')IV'1°''lae Dascrilation Analytic Method N11)1, No , .. �,. Water r Grab Nitritea l itaaaat in aa�cltavaatu EJI 353.2 J Item. ,t) l}ti(.); I C},tJ rnaatrive s as ata '.1. as Naaa .r p ? Willer t nd—) I otaal Pll0sj51ae+rac laa �'aelaaveYaas I.I';rA 36 ,1 Leta'. 0 0,01-I.O €aata 1, y matrices as alaL 'l as, 11 TKN Water Grab `I aataal Kjelelaalal Nitrogen in F-l"PA a 51.2 leer 10 O,tt anew I aelaae aaS aa'aa'lrives as Ill. 1, as N � e C 111aaa-01AIN It ra Water t:ia=aala 11lay1ralalatr�k ea€a ulalorol)I Il-,a SM 10 200 l l (aaleael.)) �� I M m, I_ {Corrected 10 }�laaaysr�la�tIan)<anel Iala.a�artalartaaa Ia�� � ea. �l7(�e;trl3l�lae�ds'Yaaae:tl'�' Dissolved Field Dissolved oxygen (DO) Discrete crete Measatrerrierit Not applicable _ t xy eaa Me.aastare ine nt concentration in � aate r nicaastared by field meter Ilei-Celli D I idd Pere�vaat DO saata€a.,atuexaa In aeaate ICI � rvty Meaastare:iaavaad Not a alal-Y1Waalale Saturation MCOSLarvaaavaat aaavaaSLUNd I o Iivle,l aaavtd° IAl I'Ielcl PH Ievel In water ane<asaared by, Discr to Mea^.astaarevaaa nt Not aplalie abler WaSUNTn011 fleld aneter 0 Slaacitic l ivld �J vciliv vex€aelaavtaaaaeaeY eal,�� ate°r Dkcrete Not Conductance N"le°asaaac€i'aeiit ncaasaarvei h\ t1eld aaavter Water Held Waresto ��eratear aaacasureal by Discrete al asaaatenaeaat ; Not alalalivaa le 1`eanpaerataare 1'wpavaasrarCtnv;a1t Halal aaaeter 0 A list of the selected canal and non-canal sites is presented in Table 2, Nvith a anal illUstrating the d stribUtiOn of these sites thrcaa.agl 0U1 the Florida Keys in Figure 5 cv cv cv LO Packet Pg. 1440 Q.7.a a, Legend 75 Sample Points U i to Values c 030 D4, i r a 044.057 a 0 F2 0-" -� 1 0 N Value CL K f <r t8 d Sl% te6 200 RM"'t tl I—�a-4—I L. 0 Figure ° Examples of values for a parameter that has a maximum value of 1 and a minimum value of c (Top) and that dataset`s corresponding interpolated surface (Bottom). ns Table 2., List q 13 block arrat,siies ,fiat• Task 2 Listen coordinates, use the IVGS 1 8-1 t1alion. All points are shown hI l i�tlt'L 5 to illustrate the distl'i1ltltiull of sits tl�tcru llc�llt tllt Keys. � ns Latitude !Longitude WSIt� Eu �, 51�e �" " Type Municlpali Location Name _ 24.67701319 81.337955 8 8075 04N Bay Task 2 t Canals � 293 BIG PINE KEY" 24.67715182 -81.38351516 6013C G4N Bay Task 2-Canals 292 LITTLE TORCH KEYCD r 24.65630897 81.2652673 8080 04S Ocean Task 2 Intact/Restored Bahia Honda 24,57349531 81,65383574 8079 02:5 Mean Task 2-Canals 475GEIGERKEY 24-78404017 -80-88589£ 7 8083 075 Ocean Task 2 Canals 164 CONCH KEY ADDED 3 LO 24.73734543 80.97775815 8082 06S Ocean Task 2-intact/Restored Marathon Curry Hammock 24.69872224 -8107367249 8081 055 Ocean Task 2-Canals Marathon 257 MARATHON � 25.33080618 -80 28597741 6001C. 14N Bay Task 2-Canals 3 OCEAN REEF CLUE 25.1646726S -80-39012985 6006A 09N Bay 'task 2--Canals 28 KEY LARGO 24,91219746 -80-70162674 8078 08N Bay Task 2 Intact/Restored Lignurnvitae Key 24.85528835 -8034503426 8078 08N Bay Task 2-Canals Islarnorada 155 LOWER MATECUMBE KEY 25 17594528 80 35268583 8087 111E Ocean Task 2 Intact/Restored rtagny Johnson � 25. 2266 80.4847034 8086 095 Ocean Task 2-Canals 84 ROCK HARBOR 1 Task 2b: Ecological (Biotic) Sampling responding to water quality 'File bentlaic CsaITUF-lUnity maps will be Used to d tc Dili ne apriori what berithic substrate arid biota should be tilr0t1e1111OLIt tilt block, array. In the field, the blocks gill e cvalLlatLd for the diversity of marine }plants and invertebrates to determine patterns from inshore to offshore, associated with specific blotopes (Nero, ?[)tl ). Surveyors will condUCt lodiN-crsity assessments at the water quality sampling points throughout the block array to Understand tile response of bcnthic CO111111 Lill itie s to water gUality. Understanding the relationship between species diversity and en ironm ntal,recologiCal PrOPCIIiCS IS Cl-LICial to evaluating and Packet Pg. 1441 Q.7.a predicting ecosystem response to changes in water quality. Various studies have focused on Biodiversity Ecosystem Functions (BEF) by utilizing different measures of ecosystem function, such as biomass production and nutrient cycling (Tilman & Downing, 1994), (Naeem, Thompson, Lawler, Lawton, & Woodfin, 1994). During the last decades,there has been increased evidence that biodiversity is strongly linked to increased stability of the ecosystem functions and enhanced Ecosystem Functions which in turn, are linked to ecosystem services (Figure 6) (Cardinale et al., 2012). Therefore, invertebrate and algae biodiversity can be used as an indicator of Ecosystem Function (and thus Ecosystem Services) for the Florida Keys canals and nearshore marine ecosystems. In Task 2b, 13 sites including canals and "non-canal" sampling grids will be surveyed twice a year (wet 2 season and dry season surveys). Within each site, a subset of the water sampling points will be surveyed, each survey point will be classified by habitat and zones. The monitoring will consist of 9 canal and 4 non-canal sites with benthic sampling carried out using the following sampling distribution,these stations are a subset of the water quality stations described in 2a: A. 2 stations in Zone 1 B. 2 stations in Zone 2 C. 2 stations in Zone 3 0 The ecological surveys will focus on the conspicuous benthos and will have two components: • Submerged Aquatic Vegetation(SAV)coverage,that will be assessed through the Braun-Blanquet method consistent with previous studies (Collado-Vides, Caccia, Boyer, & Fourqurean, 2007; Fourqurean, Durako, Hall, & Hefty, 2002; Trevathan-Tackett, Lauer, Loucks, Rossi, & Ross, 2013), and 2 • Invertebrate epifauna species assemblages that will be assessed through the point intercept method. c The surveys will be carried out using a 25m transect line along with 6 quadrats (per method) placed at random locations on the transect line within each block. Therefore,there will be an overall of 6 quadrats*2 blocks/zone*3 zones=36 quadrats per site and per method. Quadrats will be photographed to review species identification and field data entry. Species will be identified using a checklist of common and charismatic species (Appendix 1 lists invertebrates and Appendix 2 lists marine plants). LO The two components are: 1. A Braun-Blanquet assessment (Kent, 2012) of substrate and algae coverage. For each of the following categories, coverage will be assessed in a .5 x.5 in quadrat (.25 in as listed in.Table 3. Categories include: a. Bare sand or mud b. Benthic macro algae and algal turf c. Seagrass 2. A point intercept method to quantify epifauna categories in a 0.5*0.5m intercept quadrat Species richness and benthic diversity will be assessed within each site. Categories will include: a. Sponges b. Hard corals c. Soft corals and anemones d. Echinoderms Packet Pg. 1442 Q.7.a e. Mollusks f Annelids c:on plete list of the species that will be recorded was developed from historical records and research publications (Appendix 1). The Braun-131ancluet coverage of seagrass will allow comparison of seagrass density perform d on previous snrv°eys used to assess seagrass health in Florida Bay (Trevatliarl-`f"ackett, Ch f.,aucr, Loucks, Rossi, & Doss. `013). -pile focus of` the surveys will be epif'auna and will not include. in fauna auna survev's. c c Water gialit;v measurements will include turbidity assesstiaents, but PAR n-reasuren-rents will also be rnade with the I-lobo Pendants. Invertebrate biodiversity, will be assessed in terills of evenness and species y richness-species assemblages Av"il1 be conipar d between the sites and over time as per niethods ir1 SulIivanCL � Cliiappone, 1992 , Table 3: Sewing to be ttsed,bi- r°c un- Blanquet.sui ve,Ys°°„ i -� twr'e sl)ecyies that occ°to-aleer; Iiwnseet bast not in c uacirttts, + �)-weal but less than 5 gf9the quadrot c vei-O e, Braun- lanquet scale Range of cover r < 5 it very few individuals CD 5%� few individual 0 1 < 5%,,numerous indMdraals 0 5— 5% 76 25 50 —°7 5% Ca sm 75® 100 % Data will be grouped together according to the distance frorn the canal and analyses will be made 0) accordingly. For t.lae species assenibla es. an MDS and cluster-analysis will be conducted to visualize the z similarities between blocks f'roni the shore Out to 500 ill. file purpose of'the Biological Assessnient will be to assess the levels and cornposition ofspecies diversity and lint:these results with Ecological Functions of"tile ecosystem. The biodiversity assessment should provide additional support to the conclusions drawn LO CD from the water duality sampling. `specifically. the sarnphng design will address tile following questions, lea l.s thcr ca cliffe3r'enc e in water"qn alio,helit een can al.s°carrel non-c•on al.s°ites? 0) lb. is the)-c ci cTr tcr'c nce in I'VO belireen cliff�t-erjt 7017c.s°`ltctlritcats i4 itirirt the (li/;rcI-e at sites."' Pi- ac°tic°calla`. sloes elis°tcancc fi'rrtra "source"" ti.e. c°carrcali raacrttc}r; ?ca. Is there ca clijf,!i-e ace in hiodivei s•itl,be°tiveen canals anal non-c-tin al.sifeks;` y b. A 1her e ca tlrfter ence in biotliversitY beli4'een(Titer ent®ones"i"habilats within the clIjle)-enl,sites? Practically, (loos cftstcanc°c Tr rrrrr '.s°€ arrcc " rrtrrtter',' 3. Uri biarcliver'sityp alter ns align with 11"Op atte i-ns? � For this Purpose, several unty ariate and niultivariate analysis will be conducted in carder to investigate for statistical significance and correlation patterns correspondingly. For first questions, data will be grouped by sites Ox, canals vs non-canals) and the mean of each nictasurerIIettt (TN TP, 1'KN Chia) will be calculated. The set of differences when subtracting non-canal Crone canal values will then be bootstrapped and tiie raicuall value and the, 95% Confidence interval will be estimated. This way we could identify ally Packet Pg. 1443 Q.7.a statistically significant differences in WQ between canals vs non-canal sites. The data can also be grouped by the time of the year collected and can be analyzed separately to ensure that we capture any seasonal variability (i.e. separate analysis for each quarter). In addition to this, a multivariate analysis will be conducted to visualize any temporal or spatial pattern of the WQ data. An nMDS and a Cluster analysis will be conducted using data for each measurement separately. For question lb, data will be grouped by either zone or habitat and analyzed accordingly. The same set of analysis will be used, by utilizing the Ch mean measurements of each zone/habitat. Therefore, the bootstrap technique will be used by estimating the differences between different zones in pairs (e.g. zone 1- zone 2 or zone 2- zone 3). e c Following the same reasoning, biodiversity measurements (questions 2a and 2b) will also be analyzed using the bootstrap technique and NMDS and Cluster analysis. In this case, biodiversity measurements y will be further grouped by category (hard corals, soft corals etc.). 2 CL Regarding question 3, the nMDS and Cluster plots derived from questions 1 and 2 will be compared and ' any overlaying patterns will be identified. In addition, the results from all the statistical tests will also be compared. For example, using the statistical outcomes form questions la and 2a, we could observe . whether significance is present when with the same pattern (i.e. if we find significant differences in WQ between canals vs non-canal sites, we should also find significance in the corresponding biodiversity). Extreme Event Sampling: - If there is an extreme rainfall event, the team will be ready to mobilize to sample some or all of the canal grids within 48 hours of the event to understand the role of acute water quality changes with storm events. This "extreme event" sampling would occur when a pre-determined meteorological trigger is reached in terms of defined precipitation indices (Table 4)3. One extreme event sampling event would be carried out once each calendar year depending on the occurrence of such events. Precipitation indices that are used to define extreme rainfall events are likely calculated after the fact, and e consultation with meteorologists can help define the specific triggers that would justify an extreme event sampling in the Upper, Middle or Lower Keys. The triggers will likely be unique to each region of the Florida Keys Table 4: Definition of the precipitation indices used to define extreme rainfall events. Precipitation Index with definitions and units: LO PRCPTOT Annual precipitation Annual total precipitation mm SDII Simple daily intensity index Annual precipitation divided by number of wet days mm/day CDD Consecutive dry days Maximum number of consecutive dry days days CWD Consecutive wet days Maximum number of consecutive wet days days R10mm Days above 1 Omm Annual count of days when RR>1 Omm days R20mm Days above 20mm Annual count of days when RR>20mm days R50mm Days above 50mm Annual count of days when RR>50mm days y RXlday Max 1-day precipitation Annual highest daily precipitation mm RX5day Max 5-days precipitation Annual highest 5 consecutive days precipitation mm R95p Very wet days Annual total precipitation when RR>95th percentile mm s Extreme rainfall events are defined as 5 standard deviation threshold from monthly means, and represent values that are very rare and typically only exceeded in the case of a direct impact of tropical cyclone or cold front. Packet Pg. 1444 10 U01MUGSOM - !welw j0 nAq 6uijo4!uoLAj leuBD/ a0y a ) MZ/ZZ/90 4UOWPUGWV 4sL :4u8wg3B44 rc ;m U a 19 �^a r � i w ly a cu p VN exr yr 0 � , 10 U01MUGSOM - !welw j0 nAq 6uijo4!uoLAj leuBD/ a0y a ) MZ/ZZ/90 4UOWPUGWV 4sL :4u8wg3B44 ZZ ;m U a w �< < a I l ^�d pggY )t 11 fi 70 ts,1t)s 0 it Im ur CO "sue zz v 0 m u 0 o Q.7.a APPENDIX 1: Preliminary invertebrate epifauna species list for near shore benthic communities of the Florida Keys ' TAXA Binomial Common Name/Description CNIDARIA Actinoporus elegans Elegant anemone CNIDARIA Agalophenia latecarinata feather plume hydroid CNIDARIA Agaricia spp lettuce corals CNIDARIA Bartholomea annulata ringed anemone CNIDARIA Briareum asbestinum corky sea finger y CNIDARIA Cassiopea xamachana mangrove upsidedown jelly CNIDARIA Condylactis gigantea giant pink tipped anemone CNIDARIA Dichocoenia stokesi elliptical star coral c CNIDARIA Diploria clivosa knobby brain coral CNIDARIA Diploria labyrinthiformis grooved brain coral CNIDARIA Diploria strigosa symmetrical brain coral CNIDARIA Discosoma spp unknown corallimorph - CNIDARIA Erythropodium caribaeorum encrusting gorgonian CNIDARIA Eunicea spp knobby sea rods, candelabra -CNIDARIA Eusmilia fastigiata smooth flower coral CNIDARIA Favia fragum golf ball coral CNIDARIA Halocordyle disticha christmas tree hydroid CNIDARIA Lebrunia coralligens Hidden anemone CNIDARIA Lebrunia danae branching cryptic anemone CNIDARIA Manicina areolata rose coral CNIDARIA Meandrina meandrites maze coral CNIDARIA Millepora alcicornis branching/encrusting fire coral CNIDARIA Millepora complanata Blade fire coral 0 CNIDARIA Obicella (Montastaea) spp All reef-building Montastraea CNIDARIA Palythoa caribaeorum white encrusting zoanthid CNIDARIA Phymanthus crucifer beaded or flower anemone CNIDARIA Plexaura homomalla black sea rod CNIDARIA Plexaura spp Unknown sea rod N CNIDARIA Plexaurella spp slip pore sea rods LO CNIDARIA Porites asteroides mustard hill coral CNIDARIA Porites porites clubtip finger coral CNIDARIA Porites divaricata thin finger coral CNIDARIA Pseudoplexuana spp Porous sea rods E CNIDARIA Pseudopterogorgia spp. rough sea plume y CNIDARIA Siderastrea radians lesser starlet coral CNIDARIA Stephanocoenia intersepta blushing star coral CNIDARIA Stichodactyla helianthus sun anemone PORIFERA Amphimedon compressa red finger sponge (formerly H, rubens) PORIFERA Aplysina sp. Unknown Aplysina PORIFERA Callyspongia vaginallis branching vase sponge, grey-purple tube PORIFERA Chondrilla caribensis(nucula) chicken liver sponge PORIFERA Cinachyra sp. dusty orange ball sponge 16 Packet Pg. 1447 Q.7.a PORIFERA Cliona (Anthosigmella) varians (brown) variable sponge PORIFERA Cliona delitrix orange boring sponge PORIFERA Cliona langae coral encrusting sponge NOW C.apria PORIFERA Cliona sp. green velvel encrusting C. caribbaea PORIFERA Dysidea etheria heavenly sponge PORIFERA Ectyoplasia ferox orange volcano sponge PORIFERA Haliclona sp. PORIFERA Haliclona viridis small green tubes PORIFERA Ircinia felix' stinker sponge c PORIFERA Spheciospongia vesparium Florida loggerhead sponge PORIFERA Tedania ignis fire sponge, organge color Annelida Anamobaea orstedii Split-Crown Feather Duster Annelida Arenicola cristata Southern Lugworm Annelida Bispira brunnea Social Feather Duster _ Annelida Bispira variegata Variegated Feather Duster Annelida Eupolymnia crassicornis Spaghetti Worm c Annelida Hermodice carunculata Bearded Fireworm Annelida Notaulax nudicollis Brown Fanworm Annelida Notaulax occidentalis Yellow Fanworm Annelida Sabellastarte magnifica Magnificent Feather Duster Annelida Spirobranchus giganteus Christmas Tree Worm Annelida' Spirorbis spirorbis Seagrass epiphyte Chordata Ascidia nigra Black Solitary Tunicate Chordata Botrylloides nigrum Flat Tunicate Chordata Botryllus sp. Geometric Encrusting Tunicates c Chordata Clavelina sp. Bulb Tunicates Chordata Diplosoma glandulosum Globular Encrusting Tunicate W Chordata Distaplia corolla Button Tunicates Chordata Ecteinascidia turbinata Mangrove Tunicate Chordata Polyandrocarpa tumida Mottled Social Tunicate LO Chordata Polycarpa spongiabilis Giant Tunicate Chordata Rhopalaea abdominalis Reef Tunicate Chordata Symplegma viride Encrusting Social Tunicate Chordata Trididemum solidum Overgrowing Mat Tunicate Arthropoda Callinectes sp. Blue Crabs Arthropoda Paguristes erythrops Red Banded Hermit Arthropoda Pagurus sp. Hermit Crab Arthropoda Panuliris argus Caribbean Spiny Lobster Arthropoda Petrochirus diogenes Giant Hermit Echinodermata Astropecten spp Sea Stars Echinodermata Clypeaster roseaceus inflated sea biscuit Echinodermata Diadenza antillarium Echinodermata Echinaster echinoporous thorny starfish Echinodermata Echinometra lucunter rock-boring urchin 17 Packet Pg. 1448 Q.7.a Echinodermata Echinometra viridis reef urchin Echinodermata Eucidaris tribuloides slate-pencil urchin Echinodermata Holothuria spp. Sea Cucumber Echinodermata Isostichopus badionotus Three-Rowed Sea Cucumber Echinodermata Linckia guildingii common comet star Echinodermata Lytechinus variegatus Variegated Urchin Echinodermata Tripneustes ventricosus sea egg Mollusca Atrina rigida Stiff Pen Shell Mollusca Cyphoma spp. Flamingo tongue Mollusca Eustrombus gigas Queen conch c Mollusca Fasciolaria tulipa True Tulip Mollusca Lima scabra Rough Fileclam Mollusca Octopus vulgaris Common Octopus Mollusca Phalium granulatum Scotch Bonnet , Mollusca Pickfordiateuthis pulchella Grass Squid Mollusca Pinna carnea Amber Penshell Mollusca Sepioteuthis sepiodea Caribbean Reef Squid c 0 LO N N 18 Packet Pg. 1449 Q.7.a APP i llA ?: List of Marine Plans to be scored its henthic° surveys. Species are grOUpec by Green Brown and Reef macro algae, conspietuOcts cyanobacteria and sea grasses. Numbers folloxving the species are from the C..'aribbeari Marine: Plants Kerr (Littler& Littler. 2.000).. M RtNt=- PLANT SPFCIES LIST Littler& Littler Key Aeetabularia spp, 442 nady rnene saldenhae 3 10 Anadymneue stellati 3 f 0 vrainvillea spp, 382 y Batrpltura nerstedii 436 � Bry psis lwypnuida:s 342 c Firyopsis pennata 342 c ryopsis plumosa 344 � Brydpsis ramatirasa 344 � Caulerpa cupressoides .360 CL L Caulerpa rnacrophysa 362 Caulerpa imexicana 36.1 Caulerpa paspalrwides .366, Caaderpa prtilifera 36 Caulerpa pu illa 368 Caulerpa raeemnsa ` 370 Cauterpa serrulata 372 Caulerpa sertularoides 374 Caulerpa taxifiwlia 376 Caulerpa verticillata 376 Caulerpa vickersiae 378 cJ Caulerpa wvebbiana .378 Clwadoincarplwa oracilis 3 18 Chaetomorptia linum 318 Cladnplacwra catenata 320 Cladhphora sp. 320 Codium repens 354 cV Dasycladus verimicularis 436 LO l erl esia sp. 34 Dictyosphaeria cave mosa 332 � nterornorplwa spl) l lalimeda discraidea 400 E Halirnedaa inerassata 402 < Halir cda lacrimosa 404 Halimeda trai rile 404 Halimeda opuntia 406 Halimeda seabra 406 Halimeda tuna 40 Haliplsilia decipieus 480 Microdictyon nlarinvin 312 Neomeris annulata ` 8 lPeawicillus capitatus' 410 19 Packet Pg. 1450 Q.7.a Penicitlus d metosus 410 Peni illus lartrnurouxii 412 Penicillus pyriformis 412 Rhipitcephalus phoenix 418 Ud tea spp, 422 Ulva lactuca 306 Valonia macrnphysa 340 Ventric ria ventricosa J36 y Cystdseira myrica 280 Diet opteris spp `54 Dictunta spp 487 c l ietyota caribaea 260 Lnbophara variegate 68,270 � Padina spp 277 CL sar assum spp 280 Stypopndium zonate> 278 Turbinaria turbinata 290 "} } 192 194 144 c �i r ' 146 c 198 f 204 204 136 R 0 t 170 17 . } \ 1 z 58 94 CN - }` fit) LO } t 58 46 E 1 c � 110 146 E 112 s 114 _ Gf4 1 1 4 117 I 1 t.p � r �u 11( 180 } 180 � 76 20 Packet Pg. 1451 Q.7.a 4 5� 30 212 214 216210 T" 4 a z r 86 � V�77 J}f2 38 � 42 � 0 c� Sp�# 166 $. lothrix aeruginia 470 Dichothrix s p. Lyngby°asp 450 chi othrix sp, 464 Symploca h dnoides 462 Halodulc be udcttei"(wright i) 48 Syringodium tiliforme 484 76 Thalassic testudinum 482 0 c� CN N N LO 21 Packet Pg. 1452 Q.7.a REFERENCES Aronson,R.B.,Bruno,J. F.,Precht, W. F., Glynn,P. W.,Harvell, C. D., Kaufman, L., . . . Valentine,J. F. (2003). Causes of coral reef degradation. Science (New York, N.Y.), 302(5650), 1502-1504. doi:10.1 126/science.302.5650.1502b Barreras, H. J., Kelly, E. A., Kumarb, N., & Solo-Gabriele, H. M. (2019). Assessment of local and regional strategies to control bacteria levels at beaches with consideration of impacts from climate change.Marine Pollution Bulletin, 138, 11. doi:10.1016/j.marpolbul.2018.10.046 Cardinale, B. J., Duffy, J. E., Gonzalez, A., Hooper, D. U., Perrings, C., Venail, P., . . . Naeem, S. (2012). Biodiversity loss and its impact on humanity.Nature, 486, 59. doi:10.1038/nature11148 c Eutrophication in coastal canals. (1972). Marine Pollution Bulletin, 3(2), 23. doi:10.1016/0025-326x(72)90207- x Group, Marine Classification Work W. (2012). Coastal and Marine Ecological Classification Standard. (0097- 6326). DataStream Content Solutions, LLC Retrieved from 2 0. http://miami.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwY2B QMLEwMwPWDIIJaYaJScmJ 1mYmiZYpRgbApj2wN2SUYoSywhepNHcTYmBKzRN1kHVzDXH20AWVkPEFkDMX4olBU6Sg9 GYoxsCbCFr8nV cC3 iS WIsGgkGRmaJglco2lgYFJWopFUj Kwk2GRam6RamFkAuz7AQAW 3 CTT. . 22 4- 0 Kent, M. (2012). Vegetation description and data analysis : a practical approach (Second edition. ed.). Chichester, West Sussex, UK, West Sussex, UK: Wiley-Blackwell. Naeem, S., Thompson, L. J., Lawler, S. P., Lawton, J. H., & Woodfin, R. M. (1994). Declining biodiversity can alter the performance of ecosystems. Nature, 368(6473), 734-737. Nero, V. (2005). Benthic marine plant patterns in coastal environments of the Bahamas. Bahamas Journal of e Science, 12(2), 14-20. Sullivan, K. M., & Chiappone, M. (1992). A Comparison of Belt Quadrat and Species Presence/Absence Sampling of Stony Coral (Scleractinia and Milleporina) and Sponges For Evaluating Species Patterning on Patch Reefs of the Central Bahamas. Bulletin of Marine Science, 50(3), 464-464. Tilman, D., & Downing, J. A. (1994). Biodiversity and stability in grasslands.Nature, 367(6461), 363. Trevathan-Tackett; S. M., Lauer, N., Loucks, K., Rossi, A. M., & Ross, C. (2013). Assessing the relationship -- between seagrass health and habitat quality with wasting disease prevalence in the Florida Keys. Journal of Experimental Marine Biology and Ecology, 449(C), 221-229. doi:10.1016/j.jembe.2013.10.004 N Collado-Vides, L., Caccia, V. G., Boyer, J. N., & Fourqurean, J. W. (2007). Tropical seagrass-associated CLNO macroalgae distributions and trends relative to water quality. ESTUARINE COASTAL AND SHELF SCIENCE, 73(3-4), 680-694. doi:10.1016/j.eess.2007.03.009 De Carlo, E. H., Hoover, D. J.;Hoover, R. S., Young, C. W., & Mackenzie, F. T. (2007). Impact of storm runoff from tropical watersheds on coastal water quality and productivity. Applied Geochemistry, 22(8), 1777- ' 1797. doi:10.1016/j.apgeochem.2007.03.034 Fourqurean, J. W., Durako, M. D., Hall, M. O., & Hefty, L. N. (2002). Seagrass distribution in south Florida: a multi-agency coordinated monitoring program. In J. W. Porter & K. G. Porter (Eds.), The Everglades, Florida Bay, and the Coral Reefs of the Florida Keys. (pp. 497-522). Boca Raton, FL: CRC Press LLC. Lapointe, B. E., &Matzie, W. R. (1996). Effects of Stormwater Nutrient Discharges on Eutrophication Processes in Nearshore Waters of the Florida Keys.-Estuaries, 19(2), 422-435. doi:10.2307/1352460 Littler, D. S., & Littler, M. M. (2000). Caribbean Reef Plants. Washington, D.C.: Offshore Graphics. Trevathan-Tackett, S. M., Lauer, N., Loucks, K., Rossi, A. M., & Ross, C. (2013). Assessing the relationship between seagrass health and habitat quality with wasting disease prevalence in the Florida Keys. Journal of Experimental Marine Biology and Ecology, 449(C), 221-229. doi:10.1016/j.jembe.2013.10.004 22 Packet Pg. 1453 Q.7.a Wagner, D., Mielbrecht, E., & van Woesik, R. (2008). Application of Landscape Ecology to Spatial Variance of Water-Quality Parameters Along the Florida Keys Reef Tract.Bulletin of Marine Science, 83(3), 553-553. Wolanski, E., Martinez, J. A., & Richmond, R. H. (2009). Quantifying the impact of watershed urbanization on a coral reef. Maunalua Bay, Hawaii. Estuarine, Coastal and Shelf Science, 84(2), 259-268. doi:10.1016/j.ecss.2009.06.029 c c y 0 c 0 LO N N N U 23 Packet Pg. 1454 Q.7.a EXHIBIT B-1 FLORIDA KEYS WATER QUALITY MONITORING SERVICES IN MONROE COUNTY, FLORIDA DELIVERABLES FOR TASK 2 Monroe County Canal Water Quality Monitoring The University of Miami will be responsible for maintaining and managing data files and field sheets/forms for all data and observations made in the field. All field forms will be filled-out completely and properly. The contractor will maintain the original forms with copies provided to Monroe County as described below. Field measurements of DO (concentration and percent saturation), pH, specific conductance, and water temperature for the surface and bottom readings will be maintained in electronic data files. Data files from field work must include all appropriate quality control and quality assurance information and - metadata including geo-locational identifiers, project identifiers, and site identifiers. All data submitted in electronic format by the University of Miami to DEP must have undergone a rigorous c evaluation to assess content, quality, integrity and usability. Electronic data files must be submitted either as an Excel file, a comma delimited file (.csv), a comma delimited .S text file (.txt), or other format approved by the Department. c 0 Each quarter,the data from the previous quarter are to be reviewed and loaded in the WIN database. A quarterly progress report will be submitted to the County that includes the following information: (a) A tabulation of the water quality samples collected at each station during the quarter including sampling dates and times for each site c (b) Identify an field assurance problems encountered during the quarter (c) Identify any corrective actions necessary as a result of problems encountered during the quarter cv (d) Copies of all field data sheets\forms and notes for samples and measurements collected LO during the quarter (e) Technical audit reports (more info on this in Appendix 5 of grant agreement) of monitoring performed each quarter; Due: Within 30 days after first sampling event and then quarterly (within 30-days after sampling events each quarter) until sampling events completed 24 Packet Pg. 1455 Q.7.a Deliverables to be provided by the University of Miami: Component 1 —QAPP ■ Deliverable la: Draft QAPP submitted for review; Due: Before June 15, 2019 ■ Deliverable lb: Final approved QAPP; Due: No later than 10 days before first sampling event ■ Deliverable 1 c: Technical audit reports (more info on this in Appendix 5 of grant agreement) of monitoring performed each quarter; Due: 30 November 2019 Component 2—Monitoring—2 years: July 2019 through June 2021 ■ Deliverable 2: A summary of the samples collected and delivered to the lab for analysis. Summary of completed monitoring activities (dates completed, sampling conducted and any not conducted and why, monitoring results along with interpretation of those results (as expected or not as expected submitted electronically), along with the draft or final laboratory report and sampling logs; Due by 31 July 2021 but updates to be submitted quarterly. CL Component 3 —Reporting • Deliverable 3a: Draft Final Report explaining the scientific findings; Due 1 August 2021 • Deliverable 3b: Final Report with edits incorporated, etc.*; Due 15 October 2021 • Deliverable 3c: The University shall present the results to the Monroe County Board of County Commissioners. 0 0 LO N N N 25 Packet Pg. 1456 Q.7.a EXHIBIT C-1 FLORIDA KEYS WATER QUALITY MONITORING SERVICES BUDGET WORKSHEETS BY Quarter for amended TASK 2: Water Quality in Residential Canals y The University of Miami will submit invoices to Monroe County on the following dates: a 1. 30 June 2019 2. 30 September 2019 3. 31 December2019 4. 31 March 2020 5. 30 June 2020 6. 30 September 2020 CL 7. 31 December 2020 8. 31 March 2021 9. 30 June 2021 10. 15 October 2021 —END OF PROJECT c c 0 c N N N LO 26 Packet Pg. 1457 Q.7.a Exhibit C-I FLORIDA KEYS WATER QUALITY MONITORING SERVICES BUDGET WORKSHEETS BY Quarter and task. YEAR 2 2020 TASK 2 Residential Canals Water Quality Quarter 1 Quarter 2 Quarter 3 Quarter 4y INVOIC"E DATE: INVOICE DATE: INVOICE DATE: INVOICE DA,rE: � 30 June 2019 30 September 2019 31 December 2019 31 March 2020 c Deliverable Iit: Sampling events #1 Samptingevents##2 Sampling events #3 Draft QAPP submitted Deliverable 2 Deliverable 2: A Deliverable 2: Aa for review surnmary of the samples summary of the sar Ales summary of the samples Due: efiiie u11e 1 collected and delivered to collected and delivered to collected and delivered to , 2019 the lab for the lab for the lab for analysis', Summary of analysis. Summary F of analysis.. Summary of Deliverable Ib: completed monitoring completed monitoring completed monitoring Final QAPP submitted activities activities activities' Dice: 30 June 2019 Deliverable Ic: "technical audit reports WIN UPLOAD DATE: WIN UPLOAD DATE: WIN UPLOAD DATE: WIN UPLOAD DATE: none I-Nov 2019 1-Feb-2020 I-May-2020 1 ,287 663287 1563�287 $66,286 YEAR 3 2021 „..,. 0 Quarter I (quarter 2 Quarter 3 _ Quarter 4 v INVOICE, DATE INVOICE DATE INVOICE DATE: INVOICE DATE a 30 June 2020 30 September 2020 31 December 2020 31 March 2021 `-' Sampling events #4 Sampling events #5 Sampling events #6 Samplin events #7 `= N Deliverable 2: A Deliverable 2: .A Deliverable 2: .A Deliverable 2: LO suiilnlary of the samples sunlnlary of"the samples sun-iiliary of t11e samples summary of the samples collected and delivered to collected and delivered to collected and delivered to collected and delivered to 5 the lab foi- the lab for the lab for the lab ter analysis. SU111n1ary of analysis. Summary off analysis. Suniniary of analysis. Summary of a) coinplcted nionitoring completed monitoring conipleted nionitoi•inp conllilctcd nionitoring activities activities activities activities n WIN UPLOAD DATE: WIN UPLOAD DATE: WIN UPLOAD DATE: WIN UPLOAD DATE: I August 2020 I-November2t12b I-Febs202I 1-Ma -2021 S47,721 J7,721 S#7,721 471722 27 Packet Pg. 1458 Q.7.a YEAR 4 2022 Quarter 1 Quarter 2 INVOICE DATE INVOICE DATE,: 30 June 2021 30 September 2021 Sampling events #8 Deliverable Via: Deliverable 2: A Draft l Mal Report sunrn ary ul'the samples Due I August 2021 � collected and delivered to the lab for Deliverable Jb® analysis, Summary of. I'Mal Report tmth edits completed m mitoring Meorporated, etc.,) aetiVilies 11reselrtatieaur to tic rrrc e ('ouiltv BOCC: Due 15 0cwher 021 WIN UPLOAD DATE; WIN UPLOAD DATE: 1 August 2021 none 58 587.50 $32,591.50 c 0 0 N N N LO ell Packet Pg. 1459 Q.7.a i i ® DATE(MM/DO/YYYY) CERTIFICATE OF LIABILITY"INSURANCE 10/15/2018 THIS CERTIFICATE IS ISSUED AS A MATTER OF INFORMATION ONLY AND CONFERS NO RIGHTS UPON THE CERTIFICATE HOLDER. THIS CERTIFICATE DOES NOT AFFIRMATIVELY OR NEGATIVELY AMEND, EXTEND OR ALTER THE COVERAGE AFFORDED BY THE POLICIES BELOW. THIS CERTIFICATE OF INSURANCE DOES NOT CONSTITUTE A CONTRACT BETWEEN THE ISSUING INSURER(S), AUTHORIZED REPRESENTATIVE OR PRODUCER,AND THE CERTIFICATE HOLDER. IMPORTANT:If the certificate holder Is'an ADDITIONAL INSURED,the policy(ies)must have ADDITIONAL INSURED provisions or be endorsed.If SUBROGATION IS WAIVED,subject to the terms and conditions of the policy,certain policies may require an endorsement.A statement on this certificate does not confer.rights to the certificate holder in lieu of such endorsement(s). PRODUCE CONTACT R......._. - .. NAME' Aan Risk Services, inc of Florida N - FAx" "- r+- 1001 Brickell Bay Drive AJC..NaEato C666) 283-7122 AIc-No•: (800) 363-0105 0 O Suite 1100 E-MAIL - Miami FL 33131 USA ADDRESS: _ "INSURER(S)AFFORDING COVE RAGE NAIC# INSURED - INSURER A: Lloyd's syndicate No. 2003' AA1128003 University of Miami INSURERS: National Union Fire Ins CO of Pittsburgh 19445 N 1320 S. DIXIE HIGHWAY,SUITE 1200 coral Gables FL 33146 USA INSURERC: American Home-Assurance Co. 19380 CL INSURER D: New Hampshire insurance Company ., ...-_ _ 23841 , , , INSURER E: INSURER F: (B ..COVERAGES CERTIFICATE.NUMBER:570073565723, REVISION NUMBER:'. .THiSIS TO CERTIFY THATTHE'POLiCIES OF'iNSURANCE LISTED BELOW HAVE BEEN ISSUED TO THE INSURED NAMED ABOVE FOR THE POLICY PERIOD INDICATED.NOTWITHSTANDING ANY REQUIREMENT,TERM OR CONDITION OF ANY CONTRACT OR OTHER DOCUMENT WITH RESPECT TO WHICH THIS CERTIFICATE MAY BE ISSUED OR MAY PERTAIN,THE INSURANCE AFFORDED BY THE POLICIES DESCRIBED HEREIN IS SUBJECT TO ALL THE TERMS, EXCLUSIONS AND CONDITIONS OF SUCH POLICIES.LIMITS SHOWN MAY HAVE BEEN REDUCED BY PAID CLAIMS. Limits shown are as requested INSIALTR TYPE OF INSURANCE INS 'Vivo ,POLICY NUMBER .. MMIOD SUBRrYI JFAf1I1ODIYYyYl - LIMITS A X COMMERCIAL GENERAL LIABILITY CSUSAIBUZ665 115 U/ EACHOCCURRENCE $1;500,000 CD CLAIMS-MADE ❑X occuR SIR applies per policy terns & conditions -, -cEcJvbrk- $1,500,000 PiiEMlSES IR e '�. X SIR-850D•0110 MED EXP(Any one person) EXCluded PERSONALBADV INJURY' $1,500,000 N GEN'LAGGREGATE LIMITAPPLIES PER: I GENERAL AGGREGATE' S1,500,006 O I.OTHER: POLICY ❑PRC_ ❑LOC PRODUCTS-COMP16P AGO $1,500,00-0JECT .B .. .. ..- _ AUTOMOBILE LIABILITY CA 9767323' 'I0/15/20I6 10%15/2019 COMBINED SINGLE LIMB $1,500,000 (0 Fleet B e acdden) B X ANYAUTO CA 9767322 10/15/201810/15/2019 BODILY INJURY(Par person) O •` OWNED - SCHEDULED Fleet A/Physical Damage BODILY INJURY(Per acrJdent) y AUTOS ONLY AUTOS HIRE13AUTOS NON-OWNED PROPERTY DAMAGE'- ONLY AUTOS ONLY Par accident :r= UMBRELLA LUIO .00CUR EACH OCCURRENCE � W EXCESS LIAR CLAIMS-MADE AGGREGATE — DEO RETENTION (7) D WORKERS COMPENSATION AND WC0314679$9 10/15 2018 10/15 2019 X I PER oTH EMPLOYERS'LIABILITY YIN ADS STATUTE -F CV PNY PROPRIETOR f PARTNER/EXECUTIVE I - E.L.EACH ACCIDENT $1,000,000 D OFFICEMMEMBEREXCLUDED? a.NIA WC031467990 10/15/2018 10/15/2019 CV (Mandatory In NHl - AZ KY NC NJ NY PA VA VT E.L.DISEASE-EA EMPLOYEE $1,-000,000 11 yes,describe under _ _ — DESCRIPTION OF OPERATIONS below E.L.DISEASE-POLICY LIMIT' Sl,000,000 e' 'Excess WC -H-XWC5565571 10/15/2018 10/15/2019 EL Each Accident $500,000 FL EL Disease - Policy $500,00011� SIR applies per policy ter s & condi�ions EL Disease - Ea Emp $500,000°` 0) DESCRIPTION OF OPERATIONS I LOCATIONS I VEHICLES(ACORD 101,Additional Remarks Schedule,may be attached If more space Is required) Gentificate Holder is included as Additional Insured in accordance with the poll ra��L i iJ�Tty and Auto Liability policies. 1➢ 1 Pr ' Atitk4�kNT E BY - _: DATE —I6- CERTIFICATE HOLDER CANCELLATION 0) tJ SHOULD ANY OF THE ABOVE DESCRIBED POLICIES BE CANCELLED BEFORE THE EXPIRATION DATE THEREOF,NOTICE WILL BE DELIVERED IN ACCORDANCE WITH THE POLICY PROVISIONS. Monroe County AUTHORIZED REPRESENTATIVE - Board of County Commissioners 1100 Simonton street - The Gasp Building, Room 2-205 M49 Key west FL 33040 USA a. �t.ZVG�4 ©1988-2015 ACORD CORPORATION.All rights reserved. ACORD 25(2016103) The ACORD name and logo are registered marks of ACORD Packet Pg. 1460 Q.7.a ACCO® CERTIFICATE OF MARINE / ENERGY INSURANCE D07/15/2019(MMIDDIYYYY' THIS CERTIFICATE IS ISSUED AS A MATTER OF INFORMATION ONLY AND CONFERS NO RIGHTS UPON THE CERTIFICATE HOLDER. THIS CERTIFICATE DOES NOT AFFIRMATIVELY OR NEGATIVELY AMEND, EXTEND OR ALTER THE COVERAGE AFFORDED BY THE POLICIES BELOW. THIS CERTIFICATE OF INSURANCE DOES NOT CONSTITUTE A CONTRACT BETWEEN THE ISSUING INSURER(S), AUTHORIZED REPRESENTATIVE OR PRODUCER,AND THE CERTIFICATE HOLDER. IMPORTANT: If the certificate holder is an ADDITIONAL INSURED,the policy(ies) must have ADDITIONAL INSURED provisions or be endorsed. If SUBROGATION IS WAIVED,subject to the terms and conditions of the policy, certain policies may require an endorsement. A statement on this certificate does not confer rights to the certificate holder in lieu of such endorsement(s). _ CONTACT (n PRODUCER Marsh USA Inc. NAME: FAX 1560 Sawgrass Corporate Pkwy,Suite 300 PHONEI o Ext): A/C No): fry Sunrise,FL 33323 E-MAIL ADDRESS: PRODUCER CUSTOMER ID#: CN102606713—Marin-18-19 INSURERS AFFORDING COVERAGE NAIC# Ir INSURED wsuRER A:Liberty Mutual Insurance Company 23043 University of Miami Department of Risk Management INSURER B:Environmental Pollution Group 1320 South Dixie Highway INSURER C: N Suite 1200 Miami,FL 33146-2945 INSURER D: INSURER E: INSURER F: COVERAGES CERTIFICATE NUMBER: ATL-004966023-01 REVISION NUMBER:1 ce THIS IS TO CERTIFY THAT THE POLICIES OF INSURANCE LISTED BELOW HAVE BEEN ISSUED TO THE INSURED NAMED ABOVE FOR THE POLICY PERIOD INDICATED. NOTWITHSTANDING ANY REQUIREMENT, TERM OR CONDITION OF ANY CONTRACT OR OTHER DOCUMENT WITH RESPECT TO WHICH THIS CERTIFICATE MAY BE ISSUED OR MAY PERTAIN, THE INSURANCE AFFORDED BY THE POLICIES DESCRIBED HEREIN IS SUBJECT TO ALL THE TERMS, EXCLUSIONS AND CONDITIONS OF SUCH POLICIES.LIMITS SHOWN MAY HAVE BEEN REDUCED BY PAID CLAIMS. INSR ADDLSUBRTYPE OF INSURANCE INSD WVD POLICY NUMBER POLICY MM DD/YYYY MMIDD EFF IYYY LTR Y LIMITS :A HULL AND MACHINERY LIUH-00113-07 09/01/2018 09/01/2019 PER SCHEDULE ON FILE CD X INSURED VALUE g 4,200,00 . COLLISION LIABILITY COLLISION(Ea occurrence) S TOWERS LIABILITY TOWERS(Ea occurrence) S O S A PROTECTION AND INDEMNITY LIUH-00113-07 09/01/2018 09/01/2019 PER CLUB RULES X CREW LIABILITY ❑JONES ACT X EAOCCURRENCE 1,000,0( PER VESSEL CSL S X. COLLISION LIABILITY COLLISION(Ea occ),CSL S 1,000,0( X TOWERS LIABILITY TOWERS(Ea occ),CSL S 1,000,0( REMOVAL OF WRECK REMOVAL OF WRECK Ea occurrence S O IN REM g S s B POLLUTION LIABILITY 05293-14 09/01/2018 09/01/2019 EA OCCURRENCE S OPA 90 g CD CD CERCLA $ NON-OPA/NON-CERCLA s CV T Vessel Pollution LO g 2,500,0( MARITIME EMPLOYERS LIABILITY ANY ONE PERSON g ar ALTERNATE EMPLOYER Y AI =- Li T ANY ONE ACCIDENT $ E INCLUDES CREW EMPS S JONESACT NIA S DEATH ON THE HIGH SEAS DATE ' S IN REM ENDORSEMENT WAIVER W/ YES— S y P' $ $ 5 S CERTIFICATE HOLDER CANCELLATION Monroe County,Attn:Ms.Rhonda Haag SHOULD ANY OF THE DESCRIBED POLICIES BE CANCELLED BEFORE THE Sustainability&Projects EXPIRATION DATE THEREOF,NOTICE WILL BE DELIVERED IN ACCORDANCE 102050 Overseas Highway,Ste.246 WITH THE POLICY PROVISIONS. Key Largo,FL 33037 AUTHORIZED REPRESENTATIVE of Marsh USA Inc. Manashi MukherjeeoLu>zrs►�t Page 1 of 2 ©2012-2016 ACORD CORPORATION. All rights reserved. ACORD 31 (2016/03) The ACORD name and logo are registered marks of ACORD Packet Pg. 1461 Q.7.a COVERAGES CERTIFICATE PLUMBER: ATL-004966023-01 1�TR ADD U TYPE OF INSURANCE DL D POLICY NUMBER MMIDDPOLICYIYYYYI IMMIDDIYYYYI LIMITS COMMERCIAL GENERAL LIABILITY EACH OCCURRENCE g MARINE GENERAL LIABILITY DAMAGE TO R PREMISES Ea occurrence S CLAIMS-MADE OCCUR - MED EXP(Any one person) g PERSONAL&ADV INJURY g GENERAL AGGREGATE g GENT AGGREGATE LIMIT APPLIES PER: PRODUCTS-COMP/OF AGG g N POLICY JECT LOC g OTHER: N S AUTOMOBILE LIABILITY COMBINED SINGLE LIMIT ANY AUTO SCHEDULED BODILY INJURY (Per person) g O AUTOS OWNED NON-OWNED AUTOS ONLY AUTOS ONLY BODILY INJURY(Per accident) g HIRED PROPERTY DAMAGE Per accident $ t8 WORKERS COMPENSATION PER OTH- AND EMPLOYERS LIABILITY Y/N TAT ER 0) ANYPROPRIETOR/PARTNER/EXECUTIVE N E.L.(Each accident) g OFFICER/M EMBER EXCLUDED? (Mandatory in NH) E.L.DISEASE(Ea employee) g �` If yes,describe under DESCRIPTION CL OF OPERATIONS below N/A E.L.DISEASE-POLICY LIMIT g ALTERNATE EMPLOYER g USL&HENDORSEMENT s MARITIME EMPLOYERS LIABILITY g OCSL ACT O U.S.LONGSHORE&HARBOR WORKERS PER OTH- s COMPENSATION ACT STATUTE ER ALTERNATE EMPLOYER E.L.(Each accident) g MARITIME EMPLOYERS LIABILITY N/A E.L.DISEASE(Ea employee) g OCSL ACT E.L.DISEASE-ANN AGG gam` O . S . AIRCRAFT LIABILITY EACH OCCURRENCE g O OWNED AIRCRAFT AGGREGATE g NON-OWNED AIRCRAFT gcu PASSENGER LIABILITY gcu s A UMBRELLA/EXCESS LIAR/BUMBERSHOOT NYABOOTGO01 09/0112018 09/0112019 EACH OCCURRENCE g 5,000,01 2 O UMBRELLA ❑ BUMBERSHOOT AGGREGATE S X EXCESS $ cu CLAIMS MADE ❑OCCUR g 0) DED RETENTIONS g ENERGY CSL,ANY ONE CONTROL OF WELL/OPERATORS OCCURRENCE $ CD CD EXTRAEXPENSE (100%interest) CARE,CUSTODY AND CONTROL(CCC) ANY ONE OCCURRENCE 100%interest S OFFSHORE OIL AND GAS PROPERTY toto CD PLATFORMS VALUES AS SCHEDULED g PIPELINES VALUES AS SCHEDULED g 5 S ONSHORE OIL AND GAS PROPERTY OIL&GAS PROPERTY VALUES AS SCHEDULED S CONTRACTORS EQUIPMENT VALUES AS SCHEDULED g ry $ NAMED WINDSTORM CCC SHORE SHORE AGGREGATE g VESSEL(S): AS PER ATTACHED SCHEDULE AS DETAILED IN THE DESCRIPTION OF OPERATIONS U DESCRIPTION OF OPERATIONS I LOCATIONS (ACORD 101,Additional Remarks Schedule,may be attached,if more space Is required) Other certificate holder-Monroe CountyAttomey,Attn:Mr.Bob Shillinger,Post Office Box 1026,Key West,FL 33041-1026 ACORD 31 (2016/03) Page 2 of 2 Packet Pg. 1462 V) < < Z u D Z) 0 U � w LL J I- 0 cn (� o C) L w ce L - Lu < cj� Q Z `� LL Q Q — L ® cry V� L 0 LiLn Q0uj — QZ w ::) 0 LU w U QC U CL w LU O C) Z 13� 0 r' r s ■ ®' 4-j p ! 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